Protein kinase ASK1

Protein kinase ASK1, a member of the mitogen-activated protein kinase kinase kinase family, activates JNK and p38 MAP kinase signaling pathways in response to various stress stimuli, including oxidative stress, endoplasmic reticulum stress, and calcium ion influx . ASK1 plays a key role in the pathogenesis of multiple diseases including cancer, neurodegeneration and cardiovascular diseases and is considered as a promising therapeutic target. The activity of ASK1 is regulated by several other proteins including thioredoxin and the 14-3-3 protein that both function as physiological inhibitors of ASK1. Main goal of this project is to elucidtae the inhibitory mechanism of ASK1 through interaction with regulatory proteins 14-3-3 and thioredoxin (TRX1). This project was funded by Czech Science Foundation (Project 14-10061S).

Results were published in:

List of 3 publications

(3 currently shown)

S. Kylarova, D. Kosek, O. Petrvalska, K. Psenakova, P. Man, J. Vecer, P. Herman, V. Obsilova et al.
Cysteine residues mediate high-affinity binding of thioredoxin to ASK1

The FEBS Journal, 283(20), 2016

DOI, BibTeX

O. Petrvalska, D. Kosek, Z. Kukacka, Z. Tosner, P. Man, J. Vecer, P. Herman, V. Obsilova et al.
Structural Insight into the 14-3-3 Protein-dependent Inhibition of Protein Kinase ASK1 (Apoptosis Signal-regulating kinase 1)

Journal of Biological Chemistry, 291(39), 2016

DOI, BibTeX

D. Kosek, S. Kylarova, K. Psenakova, L. Rezabkova, P. Herman, J. Vecer, V. Obsilova and T. Obsil
Biophysical and Structural Characterization of the Thioredoxin-binding Domain of Protein Kinase ASK1 and Its Interaction with Reduced Thioredoxin

Journal of Biological Chemistry, 289(35), 2014

DOI, BibTeX